Non è detto che i convocati ufficiali partecipino tutti alla manifestazione, ecco l’elenco dei giocatori presenti con i sostituti degli assenti (* assente, ** sostituto). Questa edizione del Pro Bowl è quella con il minor numero di giocatori sostituiti (esclusi quelli che disputeranno il Super Bowl) dal 2017.
AFC
| RUOLO | GIOCATORE | SQUADRA |
| QB | Patrick Mahomes* (Super Bowl) | Chiefs |
| QB | Josh Allen* (infortunio) | Bills |
| QB | Joe Burrow* (altro) | Bengals |
| QB | Tyler Huntley** (sostituto) | Ravens |
| QB | Trevor Lawrence** (sostituto) | Jaguars |
| QB | Derek Carr** (sostituto) | Raiders |
| RB | Nick Chubb | Browns |
| RB | Josh Jacobs | Raiders |
| RB | Derrick Henry | Titans |
| FB | Patrick Ricard | Ravens |
| WR | Tyreek Hill | Dolphins |
| WR | Stefon Diggs | Bills |
| WR | Davante Adams | Raiders |
| WR | Ja’Marr Chase | Bengals |
| TE | Travis Kelce* (Super Bowl) | Chiefs |
| TE | Mark Andrews | Ravens |
| TE | Dawson Knox** (sostituto) | Bills |
| OT | Laremy Tunsil | Texans |
| OT | Terron Armstead | Dolphins |
| OT | Orlando Brown* (Super Bowl) | Chiefs |
| OT | Dion Dawkins** (sostituto) | Bills |
| OG | Joel Bitonio | Browns |
| OG | Quenton Nelson* (infortunio) | Colts |
| OG | Joe Thuney* (Super Bowl) | Chiefs |
| OG | Rodger Saffold** (sostituto) | Bills |
| OG | Wyatt Teller** (sostituto) | Browns |
| C | Creed Humphrey* (Super Bowl) | Chiefs |
| C | Mitch Morse | Bills |
| C | Ben Jones** (sostituto) | Titans |
| DE | Myles Garrett | Browns |
| DE | Maxx Crosby | Raiders |
| DE | Trey Hendrickson | Bengals |
| DT | Chris Jones* (Super Bowl) | Chiefs |
| DT | Quinnen Williams | Jets |
| DT | Jeffery Simmons | Titans |
| DL | Cameron Heyward** (sostituto) | Steelers |
| OLB | Matt Judon | Patriots |
| OLB | Khalil Mack* (infortunio) | Chargers |
| OLB | T.J. Watt* (infortunio) | Steelers |
| OLB | Bradley Chubb** (sostituto) | Dolphins |
| OLB | Matt Milano** (sostituto) | Bills |
| ILB | Roquan Smith | Ravens |
| ILB | C.J. Mosley | Jets |
| CB | Sauce Gardner | Jets |
| CB | Patrick Surtain II | Broncos |
| CB | Marlon Humphrey | Ravens |
| CB | Xavien Howard | Dolphins |
| FS | Minkah Fitzpatrick | Steelers |
| SS | Derwin James | Chargers |
| SS | Jordan Poyer | Bills |
| LS | Morgan Cox | Titans |
| P | Tommy Townsend* (Super Bowl) | Chiefs |
| P | A.J. Cole** (sostituto) | Raiders |
| Return Specialist | Devin Duvernay* (infortunio) | Ravens |
| Return Specialist | Jamal Agnew** (sostituto) | Jaguars |
| Special Teamer | Justin Hardee | Jets |
NFC
| RUOLO | GIOCATORE | SQUADRA |
| QB | Jalen Hurts* (Super Bowl) | Eagles |
| QB | Geno Smith | Seahawks |
| QB | Kirk Cousins | Vikings |
| QB | Jared Goff** (sostituto) | Lions |
| RB | Saquon Barkley | Giants |
| RB | Tony Pollard* (infortunio) | Cowboys |
| RB | Miles Sanders* (Super Bowl) | Eagles |
| RB | Christian McCaffrey** (sostituto) | 49ers |
| RB | Saquon Barkley | Giants |
| RB | Dalvin Cook** (sostituto) | Vikings |
| FB | Kyle Juszczyk | 49ers |
| WR | Justin Jefferson | Vikings |
| WR | A.J. Brown* (Super Bowl) | Eagles |
| WR | CeeDee Lamb | Cowboys |
| WR | Terry McLaurin | Commanders |
| WR | Amon-Ra St. Brown** (sostituto) | Lions |
| TE | George Kittle | 49ers |
| TE | T.J. Hockenson | Vikings |
| OT | Trent Williams | 49ers |
| OT | Lane Johnson* (Super Bowl) | Eagles |
| OT | Tristan Wirfs | Buccaneers |
| OT | Penei Sewell** (sostituto) | Lions |
| OG | Zack Martin | Cowboys |
| OG | Landon Dickerson* (Super Bowl) | Eagles |
| OG | Chris Lindstrom | Falcons |
| OG | Elgton Jenkins** (sostituto) | Packers |
| C | Jason Kelce* (Super Bowl) | Eagles |
| C | Frank Ragnow | Lions |
| C | Tyler Biadasz** (sostituto) | Cowboys |
| DE | Nick Bosa | 49ers |
| DE | Brian Burns | Panthers |
| DE | Demarcus Lawrence | Cowboys |
| DT | Aaron Donald | Rams |
| DT | Jonathan Allen | Commanders |
| DT | Dexter Lawrence | Giants |
| OLB | Micah Parsons | Cowboys |
| OLB | Za’Darius Smith | Vikings |
| OLB | Haason Reddick* (Super Bowl) | Eagles |
| OLB | Danielle Hunter** (sostituto) | Vikings |
| ILB | Fred Warner | 49ers |
| ILB | Demario Davis | Saints |
| CB | Darius Slay* (Super Bowl) | Eagles |
| CB | Trevon Diggs | Cowboys |
| CB | Tariq Woolen | Seahawks |
| CB | Jaire Alexander | Packers |
| CB | Jalen Ramsey** (sostituto) | Rams |
| FS | Quandre Diggs | Seahawks |
| SS | Budda Baker | Cardinals |
| SS | Talanoa Hufanga | 49ers |
| LS | Andrew DePaola | Vikings |
| P | Tress Way | Commanders |
| K | Jason Myers | Seahawks |
| Return Specialist | KaVontae Turpin | Cowboys |
| Special Teamer | Jeremy Reaves | Commanders |
